head of
- UNM Center for Molecular Discovery Director
- Contact Info
- 505-272-6892
- Websites
Sklar, Larry Director – Translational Technology & Resources
Positions
- Professor Emeritus, Department of Pathology , School of Medicine
Interests:
Signal transduction, cell adhesion, leukocyte biology
High throughput technologies for molecular assembly and drug discovery
Precision Medicine
Drug Repurposing
Combinatorial libraries for discovery
Signal transduction, cell adhesion, leukocyte biology
High throughput technologies for molecular assembly and drug discovery
Precision Medicine
Drug Repurposing
Combinatorial libraries for discovery
- Affiliation
- Publications
- Background
- Contact
- View All
Affiliation
has collaborator
- Chien, Jeremy Associate Professor in Division of Molecular Medicine
- Lee, Sam A Associate Professor
- Matlawska-Wasowska, Ksenia Faculty Member
- Oprea, Tudor I. Professor
- Wandinger-Ness, Angela Professor
Publications
selected publications
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academic article
- Pyrazole-Based Lactate Dehydrogenase Inhibitors with Optimized Cell Activity and Pharmacokinetic Properties.. Journal of medicinal chemistry. 63:10984-11011. 2020
- A Selective Ligand for Estrogen Receptor Proteins Discriminates Rapid and Genomic Signaling.. Cell chemical biology. 26:1692-1702.e5. 2019
- Autophagy, Inflammation, and Metabolism (AIM) Center in its second year.. Autophagy. :1-5. 2019
- Activation of Rho Family GTPases by Small Molecules.. ACS chemical biology. 13:1514-1524. 2018
- High-Throughput Flow Cytometry Identifies Small-Molecule Inhibitors for Drug Repurposing in T-ALL.. SLAS Discovery. 23:732-741. 2018
- Pharos: Collating protein information to shed light on the druggable genome.. Nucleic acids research. 45:D995-D1002. 2017
- Novel ABCG2 Antagonists Reverse Topotecan-Mediated Chemotherapeutic Resistance in Ovarian Carcinoma Xenografts.. Molecular cancer therapeutics. 15:2853-2862. 2016
- Inhibition of Dihydroorotate Dehydrogenase Overcomes Differentiation Blockade in Acute Myeloid Leukemia. 2016
- ----Cyclic AMP efflux inhibitors as potential therapeutic agents for leukemia.. Oncotarget. :-. 2016
- Badapple: promiscuity patterns from noisy evidence.. Journal of cheminformatics. 8:-. 2016
- Discovery of Small-Molecule Nonfluorescent Inhibitors of Fluorogen-Fluorogen Activating Protein Binding Pair.. Journal of biomolecular screening. :-. 2015
- R-ketorolac Targets Cdc42 and Rac1 and Alters Ovarian Cancer Cell Behaviors Critical for Invasion and Metastasis.. Molecular cancer therapeutics. :-. 2015
- A novel pharmacologic activity of ketorolac for therapeutic benefit in ovarian cancer patients.. Clinical cancer research : an official journal of the American Association for Cancer Research. :-. 2015
- A high-throughput phenotypic screen of cytotoxic T lymphocyte lytic granule exocytosis reveals candidate immunosuppressants.. Journal of biomolecular screening. 20:359-371. 2015
- A Pan-GTPase Inhibitor as a Molecular Probe. 2015
- FRET detection of lymphocyte function-associated antigen-1 conformational extension.. Molecular biology of the cell. 26:43-54. 2015
- Novel Activities of Select NSAID R-Enantiomers against Rac1 and Cdc42 GTPases. 2015
- Pharmaceutical screen identifies novel target processes for activation of autophagy with a broad translational potential.. Nature communications. 6:-. 2015
- Quantitative Bead-Based Flow Cytometry for Assaying Rab7 GTPase Interaction with the Rab-Interacting Lysosomal Protein (RILP) Effector Protein. 2015
- Relationship of light scatter change and cdc42-regulated actin status.. Cytometry. Part B, Clinical cytometry. :-. 2015
- The evaluation of thioridazine as a hematopoietic progenitor cell mobilizing agent in healthy human subjects.. Journal of clinical pharmacology. :-. 2014
- A high throughput flow cytometric assay platform targeting transporter inhibition.. Drug discovery today. Technologies. 12:e95-103. 2014
- Selective chemical inhibition of agr quorum sensing in Staphylococcus aureus promotes host defense with minimal impact on resistance. 2014
- An automated high-throughput cell-based multiplexed flow cytometry assay to identify novel compounds to target Candida albicans virulence-related proteins. 2014
- Carbon monoxide down-regulates α4β1 integrin-specific ligand binding and cell adhesion: a possible mechanism for cell mobilization.. BMC immunology. 15:-. 2014
- Identification of Isoxsuprine Hydrochloride as a Neuroprotectant in Ischemic Stroke through Cell-Based High-Throughput Screening. 2014
- Real-time detection of protein trafficking with high-throughput flow cytometry (HTFC) and fluorogen-activating protein (FAP) base biosensor.. Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]. 67:-. 2014
- Targeting GTPases in Parkinson's disease: comparison to the historic path of kinase drug discovery and perspectives.. Frontiers in molecular neuroscience. 7:-. 2014
- High-throughput flow cytometry compatible biosensor based on fluorogen activating protein technology.. Cytometry. Part A : the journal of the International Society for Analytical Cytology. 83:220-226. 2013
- Characterization of a Cdc42 GTPase Inhibitor and its Use as a Molecular Probe.. The Journal of biological chemistry. :8531. 2013
- Characterization of a Cdc42 protein inhibitor and its use as a molecular probe. 2013
- Fluorescent substrates for flow cytometric evaluation of efflux inhibition in ABCB1, ABCC1, and ABCG2 transporters.. Analytical biochemistry. 437:77-87. 2013
- The University of New Mexico Center for Molecular Discovery.. Combinatorial chemistry & high throughput screening. 17:256-265. 2013
- A competitive nucleotide binding inhibitor: in vitro characterization of Rab7 GTPase inhibition.. ACS chemical biology. 7:1095-1108. 2012
- A competitive nucleotide binding inhibitor: in vitro characterization of Rab7 GTPase inhibition.. ACS chemical biology. 7:1095-1108. 2012
- A Selective ATP-Binding Cassette Subfamily G Member 2 Efflux Inhibitor Revealed via High-Throughput Flow Cytometry.. Journal of biomolecular screening. :-. 2012
- A novel flow cytometric HTS assay reveals functional modulators of ATP binding cassette transporter ABCB6.. PloS one. 7:-. 2012
- Aspects of VLA-4 and LFA-1 regulation that may contribute to rolling and firm adhesion.. Frontiers in immunology. 3:-. 2012
- Cluster cytometry for high-capacity bioanalysis.. Cytometry. Part A : the journal of the International Society for Analytical Cytology. 81:419-429. 2012
- Discovery of regulators of receptor internalization with high-throughput flow cytometry.. Molecular pharmacology. 82:645-657. 2012
- Identification of a small molecule yeast TORC1 inhibitor with a multiplex screen based on flow cytometry.. ACS chemical biology. 7:715-722. 2012
- Inhibitors of V-ATPase proton transport reveal uncoupling functions of tether linking cytosolic and membrane domains of V0 subunit a (Vph1p).. The Journal of biological chemistry. 287:10236-10250. 2012
- Overview: assays for studying integrin-dependent cell adhesion.. Methods in molecular biology (Clifton, N.J.). 757:3-14. 2012
- Targeting the Transposase Domain of the DNA Repair Component Metnase to Enhance Chemotherapy.. Cancer research. 72:6200-6208. 2012
- GPR30 expression is required for the mineralocorticoid receptor-independent rapid vascular effects of aldosterone.. Hypertension (Dallas, Tex. : 1979). 57:442-451. 2011
- A miniature Couette to generate shear for flow cytometry: studying real-time modulation of intracellular calcium in monocytic cells.. Cytometry. Part A : the journal of the International Society for Analytical Cytology. 79:233-240. 2011
- Associating Drugs, Targets and Clinical Outcomes into an Integrated Network Affords a New Platform for Computer-Aided Drug Repurposing.. Molecular informatics. 30:100-111. 2011
- Discovery of very late antigen-4 (VLA-4, alpha4beta1 integrin) allosteric antagonists.. The Journal of biological chemistry. 286:5455-5463. 2011
- Drug Repurposing from an Academic Perspective.. Drug discovery today. Therapeutic strategies. 8:61-69. 2011
- Drug Repurposing from an Academic Perspective.. Drug discovery today. Therapeutic strategies. 8:61-69. 2011
- Drug Repurposing from an Academic Perspective.. Drug discovery today. Therapeutic strategies. 8:61-69. 2011
- High-throughput screen for the chemical inhibitors of antiapoptotic bcl-2 family proteins by multiplex flow cytometry.. Assay and drug development technologies. 9:465-474. 2011
- Identification of a GPER/GPR30 antagonist with improved estrogen receptor counterselectivity.. The Journal of steroid biochemistry and molecular biology. 127:358-366. 2011
- Is prolonged stem cell mobilization detrimental for hematopoiesis?. Medical hypotheses. 77:1111-1113. 2011
- Microbial efflux pump inhibition: tactics and strategies.. Current pharmaceutical design. 17:1291-1302. 2011
- Nitric oxide/cGMP pathway signaling actively down-regulates ?4?1-integrin affinity: an unexpected mechanism for inducing cell de-adhesion.. BMC immunology. 12:-. 2011
- Quantum dots for quantitative flow cytometry.. Methods in molecular biology (Clifton, N.J.). 699:67-84. 2011
- Real-time analysis of the inside-out regulation of lymphocyte function-associated antigen-1 revealed similarities to and differences from very late antigen-4.. The Journal of biological chemistry. 286:20375-20386. 2011
- Simultaneous in vitro molecular screening of protein-peptide interactions by flow cytometry, using six Bcl-2 family proteins as examples.. Nature protocols. 6:943-952. 2011
- Synthesis and characterization of iodinated tetrahydroquinolines targeting the G protein-coupled estrogen receptor GPR30.. Journal of medicinal chemistry. 53:1004-1014. 2010
- A small molecule pan-inhibitor of Ras-superfamily GTPases with high efficacy towards Rab7 2010
- High-throughput multiplex flow cytometry screening for botulinum neurotoxin type a light chain protease inhibitors.. Assay and drug development technologies. 8:37-46. 2010
- Highly efficient synthesis and characterization of the GPR30-selective agonist G-1 and related tetrahydroquinoline analogs.. Organic & biomolecular chemistry. 8:2252-2259. 2010
- Identification of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay.. Journal of biomolecular screening. 15:10-20. 2010
- Identification of inhibitors of vacuolar proton-translocating ATPase pumps in yeast by high-throughput screening flow cytometry.. Analytical biochemistry. 398:203-211. 2010
- Influence of charge on cell permeability and tumor imaging of GPR30-targeted 111in-labeled nonsteroidal imaging agents.. ACS chemical biology. 5:681-690. 2010
- Profiling a Selective Probe for RTG Branch of Yeast TORC1 Signaling Pathway 2010
- Real-time partitioning of octadecyl rhodamine B into bead-supported lipid bilayer membranes revealing quantitative differences in saturable binding sites in DOPC and 1:1:1 DOPC/SM/cholesterol membranes. 2010
- Recognition of decay accelerating factor and alpha(v)beta(3) by inactivated hantaviruses: Toward the development of high-throughput screening flow cytometry assays.. Analytical biochemistry. 402:151-160. 2010
- Selective Bcl-2 Inhibitor Probes 2010
- Selective Efflux Inhibition of ATP-binding Cassette Sub-family G Member 2 2010
- Effect of DOTA position on melanoma targeting and pharmacokinetic properties of 111In-labeled lactam bridge-cyclized alpha-melanocyte stimulating hormone peptide.. Bioconjugate chemistry. 20:2162-2168. 2009
- Detection of intracellular granularity induction in prostate cancer cell lines by small molecules using the HyperCyt high-throughput flow cytometry system.. Journal of biomolecular screening. 14:596-609. 2009
- Detection of intracellular granularity induction in prostate cancer cell lines by small molecules using the HyperCyt high-throughput flow cytometry system.. Journal of biomolecular screening. 14:596-609. 2009
- Detection of intracellular granularity induction in prostate cancer cell lines by small molecules using the HyperCyt high-throughput flow cytometry system.. Journal of biomolecular screening. 14:596-609. 2009
- Detection of intracellular granularity induction in prostate cancer cell lines by small molecules using the HyperCyt high-throughput flow cytometry system.. Journal of biomolecular screening. 14:596-609. 2009
- A novel fluorescent cross-reactive formylpeptide receptor/formylpeptide receptor-like 1 hexapeptide ligand.. Cytometry. Part A : the journal of the International Society for Analytical Cytology. 75:264-270. 2009
- A crowdsourcing evaluation of the NIH chemical probes.. Nature chemical biology. 5:441-447. 2009
- Chapter 11. Subsecond analyses of G-protein coupled-receptor ternary complex dynamics by rapid mix flow cytometry.. Methods in enzymology. 461:227-247. 2009
- Conformational mAb as a tool for integrin ligand discovery.. Assay and drug development technologies. 7:507-515. 2009
- Discovery of selective probes and antagonists for G-protein-coupled receptors FPR/FPRL1 and GPR30.. Current topics in medicinal chemistry. 9:1227-1236. 2009
- Duplex high-throughput flow cytometry screen identifies two novel formylpeptide receptor family probes.. Cytometry. Part A : the journal of the International Society for Analytical Cytology. 75:253-263. 2009
- High-content screening: flow cytometry analysis.. Methods in molecular biology (Clifton, N.J.). 486:151-165. 2009
- In vivo effects of a GPR30 antagonist.. Nature chemical biology. 5:421-427. 2009
- Real-time analysis of conformation-sensitive antibody binding provides new insights into integrin conformational regulation.. The Journal of biological chemistry. 284:14337-14346. 2009
- A multifunctional androgen receptor screening assay using the high-throughput Hypercyt flow cytometry system.. Cytometry. Part A : the journal of the International Society for Analytical Cytology. 73:390-399. 2008
- Estrogen signaling through the transmembrane G protein-coupled receptor GPR30.. Annual review of physiology. 70:165-190. 2008
- Flow cytometry for real-time measurement of guanine nucleotide binding and exchange by Ras-like GTPases.. Analytical biochemistry. 381:258-266. 2008
- GPR30: a novel therapeutic target in estrogen-related disease.. Trends in pharmacological sciences. 29:116-123. 2008
- High-throughput screening for daunorubicin-mediated drug resistance identifies mometasone furoate as a novel ABCB1-reversal agent.. Journal of biomolecular screening. 13:185-193. 2008
- Preclinical development of a neutral, estrogen receptor-targeted, tridentate 99mTc(I)-estradiol-pyridin-2-yl hydrazine derivative for imaging of breast and endometrial cancers.. Journal of nuclear medicine : official publication, Society of Nuclear Medicine. 49:978-986. 2008
- The ins and outs of GPR30: a transmembrane estrogen receptor.. The Journal of steroid biochemistry and molecular biology. 109:350-353. 2008
- Synthetic estrogen derivatives demonstrate the functionality of intracellular GPR30.. ACS chemical biology. 2:536-544. 2007
- High-throughput flow cytometry for drug discovery.. Expert opinion on drug discovery. 2:685-696. 2007
- GPR30: A G protein-coupled receptor for estrogen.. Molecular and cellular endocrinology. 265-266:138-142. 2007
- Distribution and characterization of estrogen receptor G protein-coupled receptor 30 in the rat central nervous system.. The Journal of endocrinology. 193:311-321. 2007
- Rapid-mix flow cytometry measurements of subsecond regulation of G protein-coupled receptor ternary complex dynamics by guanine nucleotides.. Analytical biochemistry. 371:10-20. 2007
- Some mechanistic insights into GPCR activation from detergent-solubilized ternary complexes on beads.. Advances in protein chemistry. 74:95-135. 2007
- Spectroscopic characterization of streptavidin functionalized quantum dots.. Analytical biochemistry. 364:193-203. 2007
- The development of quantum dot calibration beads and quantitative multicolor bioassays in flow cytometry and microscopy.. Analytical biochemistry. 364:180-192. 2007
- Linkage effects on binding affinity and activation of GPR30 and estrogen receptors ERalpha/beta with tridentate pyridin-2-yl hydrazine tricarbonyl-Re/(99m)Tc(I) chelates.. Journal of the American Chemical Society. 128:14476-14477. 2006
- Steroid-binding G-protein-coupled receptors: new drug discovery targets for old ligands.. Expert opinion on drug discovery. 1:137-150. 2006
- Screening for caspase-3 inhibitors: a new class of potent small-molecule inhibitors of caspase-3.. Journal of biomolecular screening. 11:277-285. 2006
- Biomolecular screening of formylpeptide receptor ligands with a sensitive, quantitative, high-throughput flow cytometry platform.. Nature protocols. 1:59-66. 2006
- Glutathione-S-transferase-green fluorescent protein fusion protein reveals slow dissociation from high site density beads and measures free GSH.. Cytometry. Part A : the journal of the International Society for Analytical Cytology. 69:326-334. 2006
- Modulation of GPCR conformations by ligands, G-proteins, and arrestins.. Ernst Schering Foundation symposium proceedings. :211-228. 2006
- Virtual and biomolecular screening converge on a selective agonist for GPR30.. Nature chemical biology. 2:207-212. 2006
- A transmembrane intracellular estrogen receptor mediates rapid cell signaling.. Science (New York, N.Y.). 307:1625-1630. 2005
- Inhibition of chemoattractant N-formyl peptide receptor trafficking by active arrestins.. Traffic (Copenhagen, Denmark). 6:87-99. 2005
- ATP hydrolysis-dependent disassembly of the 26S proteasome is part of the catalytic cycle.. Cell. 121:553-565. 2005
- Dissociation of I domain and global conformational changes in LFA-1: refinement of small molecule-I domain structure-activity relationships.. Biochemistry. 44:4322-4331. 2005
- Dynamics of fluorescence dequenching of ostrich-quenched fluorescein biotin: a multifunctional quantitative assay for biotin.. Analytical biochemistry. 342:221-228. 2005
- High-throughput screening with HyperCyt flow cytometry to detect small molecule formylpeptide receptor ligands.. Journal of biomolecular screening. 10:374-382. 2005
- Integration of virtual screening with high-throughput flow cytometry to identify novel small molecule formylpeptide receptor antagonists.. Molecular pharmacology. 68:1301-1310. 2005
- Post-high-throughput screening analysis: an empirical compound prioritization scheme.. Journal of biomolecular screening. 10:419-426. 2005
- Small-volume rapid-mix device for subsecond kinetic analysis in flow cytometry.. Cytometry. Part A : the journal of the International Society for Analytical Cytology. 67:37-44. 2005
- Techniques: GPCR assembly, pharmacology and screening by flow cytometry.. Trends in pharmacological sciences. 25:663-669. 2004
- Real-time analysis of ternary complex on particles: direct evidence for partial agonism at the agonist-receptor-G protein complex assembly step of signal transduction. 2004
- High-throughput microfluidic mixing and multiparametric cell sorting for bioactive compound screening.. Journal of biomolecular screening. 9:103-111. 2004
- An investigation of liquid carryover and sample residual for a high-throughput flow cytometer sample delivery system.. Analytical chemistry. 76:3810-3817. 2004
- Conformational regulation of alpha 4 beta 1-integrin affinity by reducing agents. "Inside-out" signaling is independent of and additive to reduction-regulated integrin activation.. The Journal of biological chemistry. 279:32435-32443. 2004
- Flow cytometry for high-throughput, high-content screening.. Current opinion in chemical biology. 8:392-398. 2004
- Near-simultaneous and real-time detection of multiple analytes in affinity microcolumns.. Analytical chemistry. 76:6266-6273. 2004
- Alpha4beta1 integrin affinity changes govern cell adhesion. 2003
- Functional capabilities of an N-formyl peptide receptor-G(alpha)(i)(2) fusion protein: assemblies with G proteins and arrestins.. Biochemistry. 42:7283-7293. 2003
- High throughput screening of G-protein coupled receptors via flow cytometry.. Combinatorial chemistry & high throughput screening. 6:389-397. 2003
- N-formyl peptide receptor phosphorylation domains differentially regulate arrestin and agonist affinity. 2003
- FRET detection of cellular alpha4-integrin conformational activation.. Biophysical journal. 85:3951-3962. 2003
- Ligand-receptor-G-protein molecular assemblies on beads for mechanistic studies and screening by flow cytometry.. Molecular pharmacology. 64:1227-1238. 2003
- Release of ubiquitin-charged Cdc34-S - Ub from the RING domain is essential for ubiquitination of the SCF(Cdc4)-bound substrate Sic1.. Cell. 114:611-622. 2003
- Performance of in-line microfluidic mixers in laminar flow for high-throughput flow cytometry.. BioTechniques. 33:220-226. 2002
- Arrestin variants display differential binding characteristics for the phosphorylated N-formyl peptide receptor carboxyl terminus. 2002
- Biomolecular recognition on well-characterized beads packed in microfluidic channels.. Analytical chemistry. 74:1149-1156. 2002
- Flow cytometric analysis of ligand-receptor interactions and molecular assemblies.. Annual review of biophysics and biomolecular structure. 31:97-119. 2002
- Partial phosphorylation of the N-formyl peptide receptor inhibits G protein association independent of arrestin binding.. The Journal of biological chemistry. 276:49195-49203. 2001
- Real time analysis of the affinity regulation of alpha 4-integrin. The physiologically activated receptor is intermediate in affinity between resting and Mn(2+) or antibody activation.. The Journal of biological chemistry. 276:48670-48678. 2001
- Regulation of formyl peptide receptor agonist affinity by reconstitution with arrestins and heterotrimeric G proteins.. The Journal of biological chemistry. 276:49204-49212. 2001
- Real-time analysis of G protein-coupled receptor reconstitution in a solubilized system.. The Journal of biological chemistry. 276:22453-22460. 2001
- HTPS flow cytometry: a novel platform for automated high throughput drug discovery and characterization.. Journal of biomolecular screening. 6:83-90. 2001
- Plug flow cytometry extends analytical capabilities in cell adhesion and receptor pharmacology.. Cytometry. 43:211-216. 2001
- High throughput flow cytometry.. Cytometry. 44:83-90. 2001
- Analysis of free intracellular calcium by flow cytometry: multiparameter and pharmacologic applications.. Methods (San Diego, Calif.). 21:221-230. 2000
- Solubilization and display of G protein-coupled receptors on beads for real-time fluorescence and flow cytometric analysis.. BioTechniques. 28:976-80, 982-5. 2000
- Expression of P-selectin at low site density promotes selective attachment of eosinophils over neutrophils.. Journal of immunology (Baltimore, Md. : 1950). 165:404-410. 2000
- The uncoupled state of the human formyl peptide receptor.. Journal of receptor and signal transduction research. 19:327-340. 1999
- Multiple activation steps of the N-formyl peptide receptor.. Biochemistry. 38:2240-2247. 1999
- Plug flow cytometry: An automated coupling device for rapid sequential flow cytometric sample analysis.. Cytometry. 37:156-159. 1999
- Strategies for positioning fluorescent probes and crosslinkers on formyl peptide ligands.. Journal of receptor and signal transduction research. 18:187-221. 1998
- Thymic overexpression of Ttg-1 in transgenic mice results in T-cell acute lymphoblastic leukemia/lymphoma.. Molecular and cellular biology. 12:4186-4196. 1992
- Diazo coupling method for covalent attachment of proteins to solid substrates.. Bioconjugate chemistry. 17:359-365.
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article
- Autophagy, Inflammation, and Metabolism (AIM) Center of Biomedical Research Excellence: supporting the next generation of autophagy researchers and fostering international collaborations.. Autophagy. 925-929. 2018
- Advancing Biological Understanding and Therapeutics Discovery with Small-Molecule Probes.. 1252-1265. 2015
- Flow Cytometry: Impact on Early Drug Discovery.. Journal of biomolecular screening. -. 2015
Background
education and training
- Ph.D. in Chemistry, Stanford University
- Ph.D. in Chemistry, Stanford University 1972 - 1976
- The Methodist Hospital , Department of Medicine 1977 - 1979
- University of California, Santa Cruz , Division of Natural Sciences 1976 - 1977
Contact
full name
- Larry Sklar
- G M Sclar