High-throughput screening with HyperCyt flow cytometry to detect small molecule formylpeptide receptor ligands. Academic Article uri icon

start page

  • 374

end page

  • 382

abstract

  • High-throughput flow cytometry (HTFC), enabled by faster automated sample processing, represents a promising high- content approach for compound library screening. HyperCyt is a recently developed automated HTFC analysis system by which cell samples are rapidly aspirated from microplate wells and delivered to the flow cytometer. The formylpeptide receptor (FPR) family of G protein-coupled receptors contributes to the localization and activation of tissue-damaging leukocytes at sites of chronic inflammation. Here, the authors describe development and application of an HTFC screening approach to detect potential anti-inflammatory compounds that block ligand binding to FPR. Using a homogeneous no-wash assay, samples were routinely processed at 1.5 s/well (approximately 2500 cells analyzed/sample), allowing a 96-well plate to be processed in less than 2.5 min. Assay sensitivity and accuracy were validated by detection of a previously documented active compound with relatively low FPR affinity (sulfinpyrazone, inhibition constant [K(i)]=14 microM) from among a collection of 880 compounds in the Prestwick Chemical Library. The HyperCyt system was therefore demonstrated to be a robust, sensitive, and highly quantitative method with which to screen lead compound libraries in a 96-well format.

date/time value

  • 2005

Digital Object Identifier (DOI)

  • 10.1177/1087057105274532

PubMed Identifier

  • 15964939

volume

  • 10

number

  • 4

keywords

  • Flow Cytometry
  • Humans
  • Ligands
  • Receptors, Formyl Peptide
  • Sensitivity and Specificity
  • U937 Cells