A high throughput flow cytometric assay platform targeting transporter inhibition. Academic Article Review uri icon

start page

  • e95

end page

  • 103

abstract

  • This review highlights the concepts, recent applications and limitations of High Throughput Screening (HTS) flow cytometry-based efflux inhibitory assays. This platform has been employed in mammalian and yeast efflux systems leading to the identification of small molecules with transporter inhibitory capabilities. This technology offers the possibility of substrate multiplexing and may promote novel strategies targeting microbial efflux systems. This platform can generate a comprehensive dataset that may support efforts to map the interface between chemistry and transporter biology in a variety of pathogenic systems.

date/time value

  • June 2014

Digital Object Identifier (DOI)

  • 10.1016/j.ddtec.2014.03.010

PubMed Identifier

  • 25027381

volume

  • 12

number

keywords

  • ATP-Binding Cassette Transporters
  • Animals
  • Biological Transport
  • Drug Discovery
  • Flow Cytometry
  • High-Throughput Screening Assays
  • Humans
  • Small Molecule Libraries
  • Substrate Specificity