Relationship of light scatter change and cdc42-regulated actin status.
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Background: Cdc42 GTPase has important roles in regulating intracellular actin reorganization. The current methods to monitor actin changes are typically complex and point by point. Methods: The effects of Cdc42 inhibitors on the side scatter changes were tested in a newly developed continuous assay using the flow cytometer. Staining with fluorescently labeled phalloidin was used for comparison. Results: Cdc42 specific inhibitors caused dose-dependent changes of both the right angle side scatter and the phalloidin stained-actin. Conclusions: The right angle light scatter change can be used as a method to circumvent phalloidin staining and be an early convenient step in screening Cdc42 inhibitors. This article is protected by copyright. All rights reserved.© 2015 International Clinical Cytometry Society.