Association studies for next-generation sequencing. Academic Article uri icon

start page

  • 1099

end page

  • 1108


  • Genome-wide association studies (GWAS) have become the primary approach for identifying genes with common variants influencing complex diseases. Despite considerable progress, the common variations identified by GWAS account for only a small fraction of disease heritability and are unlikely to explain the majority of phenotypic variations of common diseases. A potential source of the missing heritability is the contribution of rare variants. Next-generation sequencing technologies will detect millions of novel rare variants, but these technologies have three defining features: identification of a large number of rare variants, a high proportion of sequence errors, and a large proportion of missing data. These features raise challenges for testing the association of rare variants with phenotypes of interest. In this study, we use a genome continuum model and functional principal components as a general principle for developing novel and powerful association analysis methods designed for resequencing data. We use simulations to calculate the type I error rates and the power of nine alternative statistics: two functional principal component analysis (FPCA)-based statistics, the multivariate principal component analysis (MPCA)-based statistic, the weighted sum (WSS), the variable-threshold (VT) method, the generalized T(2), the collapsing method, the CMC method, and individual tests. We also examined the impact of sequence errors on their type I error rates. Finally, we apply the nine statistics to the published resequencing data set from ANGPTL4 in the Dallas Heart Study. We report that FPCA-based statistics have a higher power to detect association of rare variants and a stronger ability to filter sequence errors than the other seven methods.

date/time value

  • 2011

Digital Object Identifier (DOI)

  • 10.1101/gr.115998.110

PubMed Identifier

  • 21521787


  • 21


  • 7


  • Angiopoietins
  • Computational Biology
  • Computer Simulation
  • Databases, Genetic
  • Genetic Variation
  • Genetics, Population
  • Genome, Human
  • Genome-Wide Association Study
  • Genotype
  • Humans
  • Models, Biological
  • Models, Statistical
  • Multivariate Analysis
  • Phenotype
  • Sequence Analysis, DNA