Regulation of tau pathology by the microglial fractalkine receptor.
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Overview
abstract
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Aggregates of the hyperphosphorylated microtubule-associated protein tau (MAPT) are an invariant neuropathological feature of tauopathies. Here, we show that microglial neuroinflammation promotes MAPT phosphorylation and aggregation. First, lipopolysaccharide-induced microglial activation promotes hyperphosphorylation of endogenous mouse MAPT in nontransgenic mice that is further enhanced in mice lacking the microglial-specific fractalkine receptor (CX3CR1) and is dependent upon functional toll-like receptor 4 and interleukin-1 (IL-1) receptors. Second, humanized MAPT transgenic mice lacking CX3CR1 exhibited enhanced MAPT phosphorylation and aggregation as well as behavioral impairments that correlated with increased levels of active p38 MAPK. Third, in vitro experiments demonstrate that microglial activation elevates the level of active p38 MAPK and enhances MAPT hyperphosphorylation within neurons that can be blocked by administration of an interleukin-1 receptor antagonist and a specific p38 MAPK inhibitor. Taken together, our results suggest that CX3CR1 and IL-1/p38 MAPK may serve as novel therapeutic targets for human tauopathies.Copyright © 2010 Elsevier Inc. All rights reserved.
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Research
keywords
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Analysis of Variance
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Animals
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Animals, Newborn
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Antigens, CD
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Antigens, Differentiation, Myelomonocytic
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Cell Count
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Cells, Cultured
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Coculture Techniques
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Culture Media, Conditioned
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Disease Models, Animal
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Enzyme Inhibitors
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Gene Expression Regulation
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Humans
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Imidazoles
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Lipopolysaccharides
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Maze Learning
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Microglia
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Models, Biological
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Neurons
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Phosphorylation
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Pyridines
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Receptors, Chemokine
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Receptors, Interleukin-1
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Silver Staining
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Statistics, Nonparametric
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Tauopathies
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Time Factors
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Toll-Like Receptor 4
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p38 Mitogen-Activated Protein Kinases
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tau Proteins
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