abstract

• P21 is a secreted protein expressed in all developmental stages of Trypanosoma cruzi. The aim of this study was to determine the effect of the recombinant protein based on P21 (P21-His(6)) on inflammatory macrophages during phagocytosis.Our results showed that P21-His(6) acts as a phagocytosis inducer by binding to CXCR4 chemokine receptor and activating actin polymerization in a way dependent onthe PI3-kinase signaling pathway.Thus, our results shed light on the notion that native P21 is a component related to T. cruzi evasion from the immune response and that CXCR4 may be involved in phagocytosis. P21-His(6) represents an important experimental control tool to study phagocytosis signaling pathways of different intracellular parasites and particles.

authors

• Rodrigues, Adele A
• Clemente, Tatiana M
• Dos Santos, Marlus A
• Machado, Fabrício Castro
• Gomes, Rafael G B
• Moreira, Heline Hellen T
• Cruz, M A
• Brígido, Paula C
• Dos Santos, Paulo C F
• Martins, Flávia A
• Bahia, Diana
• Maricato, Juliana T
• Janini, Luiz M R
• Reboredo, Eduardo H
• Mortara, Renato Arruda
• da Silva, Claudio Vieira

publication date

• January 1, 2012

keywords

• Animals
• Mice
• Mice, Inbred C57BL
• Phagocytosis
• Protein Binding
• Protozoan Proteins
• Receptors, CXCR4
• Recombinant Proteins
• Trypanosoma cruzi

Digital Object Identifier (DOI)

• https://doi.org/10.1371/journal.pone.0051384

PubMed ID

• 23251513

start page

end page

volume

• 7

number

• 12