An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells. Academic Article uri icon

abstract

  • Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, in vitro enzymology, and biological application of STS-3, an activity-based fluorescent sensor for the SULT1A1 isoform.This journal is © The Royal Society of Chemistry.

publication date

  • March 2021